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Some protocols commonly used in the Towers lab

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  Publication Advice Guide - Record Keeping

  Publication Advice Guide - Record Keeping

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• Retrovirus prep by transfection of 293Ts

  Retrovirus prep by transfection of 293Ts

• Removal of Plasmid DNA from virus preps by DNAse treatment

  Removal of Plasmid DNA from virus preps by DNAse treatment

• Virus purification through 25% sucrose

  Virus purification through 25% sucrose

• Preparation of Competent Bacteria

  Preparation of Competent Bacteria

• Intracellular staining of human NF-kB in adherent cells

  Intracellular staining of human NF-kB in adherent cells

We use Bluetractor Software's for DNA analysis, oligo design, plasmid sequence management and plasmid maps.

We use the High Definition Cell Imaging technology for HighÌýContent Screening.