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User Guides:听
Start-up & shutdown & Objective cleaning.
System Overview:
Upright multiphoton confocal with water dipping long working distance objectives.
7 laser lines for excitation:听405;听458;听488;听514;听561;听594听and听633 nm.
SpectraPhysics Mai Tai eHP DeepSee multiphoton laser. 690-1040nm.
AiryscanFast for 1.7 fold improved resolution 8x improved Signal to noise.
(听or ask听Dale听if you are not sure which dye to use, 2-photon excitation doesn't work well for some fluorophores, such as Alexa647).
Objectives:
- 20x/NA1.0 Plan Apochromat Water dipping objective with correction collar. WD 2.4mm. Amazing working distance given the very high NA. Perfect for live (multiphoton) imaging due to exceptional brightness and resolution. Correction collar for RI differences from 1.33 to 1.36. Also suitable for cleared samples, particularly ScaleA2.
- 10x/NA0.5 W-Plan Apochromat Water dipping objective. WD 3.7mm.听UV VIS IR objective with amazing working distance given the high NA. Perfect for live imaging and multiphoton imaging or ablations due to exceptional brightness and sensitivity.听
- 10x/NA0.3 EC Plan-Neofluar DIC Dry. WD 5.2mm.听Large field of view and very long working distance for deep imaging with standard or 2-photon excitation. Lower NA than the 10x water dipping objective so refractive index mis-match isn't such a problem, and gives a much thicker z slice in multiphoton imaging.
- 2.5x/NA0.085 Plan-Neofluar Dry. WD 8.8mm.听Low mag long working distance objective for orienting and manipulating large samples before imaging at higher mag. Suprisingly good imaging objective for large FOV samples.
- 63x/NA1.4 Plan Apo Oil听Highest resolution Airyscan imaging available on request. Use with levelling stage.
- 5x/NA0.16 EC Plan-Neofluar. WD 18mm. Low mag long working distance objective for orienting and manipulating large samples before imaging at higher mag. Suprisingly good imaging objective for large FOV samples.
Detectors:
Non Descanned Detectors (Multi-photon) 2 x GaAsP plus 2 standard PMT NDD detectors with filter sets for blue (485 SP), green (500-550 BP), red (575-610 BP) and far red (640-710 BP)
Descanned Detectors (Single photon) 1 x GaAsP plus 2 standard PMT detectors
Features:
- Super-resolution confocal imaging with AiryscanFast
- Deep tissue imaging (RI matched objectives, 2 photon)
- Ablations and dissection with 2-photon laser
- GaAsP internal and external detectors for highest sensitivity
- Z sectioning for 3D imaging
- Transmitted light detector (DIC objectives)
- FRET and FRAP
- Live imaging (CO2, O2, humidity and temperature controlled chamber)
- Multi-point experiments听 (XYZ positions, tiling)
- Variable stage speed for slow movement of delicate samples
Cost:
- User operation 拢28 per hour, reduced rate 拢20 at weekends
- 拢35 per hour Non-ICH academic rate, 拢25 weekends
- User operation 拢10 per hour of overnight time-lapse
- Staff operation 拢50 per hour
- Training session 拢50 per hour
