香港六合彩中特网

In a new paper in , Professer Francesco Saverio Tedesco (香港六合彩中特网 Cell and Developmental Biology) et al聽detail the modular 3D bioengineering of multilineage skeletal muscles from human induced pluripotent stem cells, which are 铿乺st differentiated into myogenic, neural and vascular progenitor cells and then combined within 3D hydrogels under tension to generate an aligned myo铿乥er scaffold containing vascular networks and motor neurons.

3D bioengineered muscles recapitulate morphological and functional features of human skeletal muscle, including establishment of a pool of cells expressing muscle stem cell markers. Importantly, bioengineered muscles provide a high-铿乨elity platform to study muscle pathology, such as emergence of dysmorphic nuclei in muscular dystrophies caused by mutant lamins. The protocol is easy to follow for operators with cell culture experience and takes between 9 and 30 d, depending on the number of cell lineages in the construct. They聽also provide examples of applications of this advanced platform for testing gene and cell therapies in vitro, as well as for in vivo studies, providing proof of principle of its potential as a tool to develop next-generation neuromuscular or musculoskeletal therapies

Original research paper (free access):聽